Structural biology of membrane proteins: from expression, to crystallization and serial crystallography by the IBS and ESRF (7th-9th December 2015)
In the frame of Nanomem, 2 teams from IBS and ESRF both members of this Marie-Curie ITN network, organized a 3-days course addressed to the PhD students of the European network and also to the students and young researchers of the site. The course took place from the 7th to the 9th of December. The first day was devoted to the presentation of several expression systems followed by a discussion, and to purification. The 2nd day happened on ID13 where students could experience serial crystallography using microcrystals of thaumatin, GLIC, and ubiquitin. Students also visited the EMBL crystallization platform and discovered the new developments for automatic crystal harvesting. On the 3rd day, the importance of the stability of membrane proteins for functional and structural studies was highlighted and several strategies to enhance stability were presented. A presentation of the new possibilities offered by electron microscopy with a visit to the microscopes ended the session. One of the speakers, scientist in a biotech company also nicely showed in his presentation how research activities are possible in private companies. Up to 12 students followed the course including 6 from the Nanomem network.
M. Burghammer (ESRF)
H. Nury, E. Pebay-Peyroula, S. Ravaud (IBS)
The first training course at Diamond Light Source, UK (2013/10/29-2013/10/31).
Everybody is working very hard to improve their soft skills.
But there was also a chance to put on a labcoat and crack some lipidic cubic phase plates.
It was spooky at the I24 beamline with spiders, evil pumpkins and pictures of falling people.
But our students could not be scared with fake cobwebs and such and took control over the hutch.
Big thanks to Isabel and the Diamond crew for organizing this fantastic training course!
NanoMem Training by Molecular Dimensions and Swissci in the UK (2015/10/19-2015/10/23).
Wenting builds a crystal molecule by molecule, it may take some time, one has to be patient.
Rebecka analyses critically the building instructions.
Rob works just too fast for an ordinary camera.
Rob's finger got stuck in his construction illustrating the drawbacks of hasty work.
Katharina is concerned about the commercial value of these constructs, maybe for a good reason. The good news is that Rob's finger is safe.
NanoMem Training at the Paul Scherrer Institute in Switzerland (2014/11/24-2014/11/28).
Anastasya looks at the sky and applaudes when seeing diffraction.
The orange and yellow team have yet another tunnel vision. Notice that Rob earned the honor of wearing the orange hat.
Valerie (second from the right) is gratefully acknowledged for organizing this memorable training course!