6th Nanomem Course - Structural biology of membrane proteins: from expression, to crystallization and serial crystallography

Grenoble, 7-8-9 December 2015

The course in Grenoble is organized by partners 5 (E.Pebay-Peyroula and her team) and 8 (M. Burghammer). It is centered on 2 main topics, membrane protein production and stabilization of the proteins for structural studies, and serial crystallography on synchrotrons. The meeting starts the 7/12 at 9h and ends the 9/12 at 12h45. Participants should arrive the 6/12 in the evening.

Arrival on the 6th of December

Monday, 7th December at IBS

9h-15h Expression (with lunch break)

Several expression systems that have been successful for membrane proteins will be presented. Their

advantages but also their limits will be discussed.

Cell free  (Viviana Job), E. coli (including fusion and refolding) (Corinne Vivès), Mammalian cells (Hugues

Nury), Yeast (Patrice Catty), Insect cells (Orso Subrini).

15h30-17h Purification

Practical training based on data which were already collected: How to conduct and to assess the different

steps of a membrane protein purification, how to characterize the purified protein (Stéphanie Ravaud)

Tuesday, 8th December at ESRF beamline ID13

9h-16h Introduction to serial crystallography (Manfred Burghammer)

Experimental set-up and sample environment, how to adapt serial crystallography to synchrotrons

16h-17h Visit to the automatic crystal fishing at EMBL (Josan Marquès)

Wednesday, 9th December at IBS

9h-11h30 Stabilization of membrane proteins in different environments

Amphipol classical or fluorinated, nanodiscs and others (Cécile Breyton)

Nanobodies (Gherici Hassaine)

11h45-12h45 Alternative approaches: Structures solved by Electron Microscopy

Presentation, visit and discussion, future perspectives (Guy Schoehn or Hélène Malet)

End of the meeting 12h45

Lecture slides: Cellfree, E.coli, Mammalian, Nanobodies, Stabilization, Yeast, Baculo

 

5th Nanomem Course - Commercialisation of Science 19th - 23th of October, 2015

Monday 19th October

Morning: arrivals at London Stansted Airport - The Stansted Airport Hilton

The hotel is connected to the terminal by a short walk way at the left hand end of the terminal as you come out of arrivals.

Lunch

Afternoon: An introduction to Intellectual Property (IP)

Alasdair Poore,Partner, Mills & Reeve LLP, Cambridge.

Dinner

Tuesday 20th October

Morning: An Introduction to Financial Management

John McManus, IfM Education and Consultancy Services, Institute for Manufacturing

Lunch

Afternoon: Establishing Strategic Priorities

John McManus, IfM Education and Consultancy Services, Institute for Manufacturing

Dinner

Wednesday 21st October

Morning: Project Management: Prioritising projects

Nicky Athanassopoulou, IfM Education and Consultancy Services, Institute for Manufacturing

Lunch

Afternoon: Project Management: Developing a Project Management Process

Nicky Athanassopoulou, IfM Education and Consultancy Services, Institute for Manufacturing

Thursday 22nd October

Morning: Writing a Business Plan: The Situation Analysis

Tony Savill, President, Molecular Dimensions Ltd.

Lunch

Afternoon: Writing a Business Plan: Getting to What, Who & When

And of course –How Much$$$$$?

Friday 23th October

2 + 2 = 5: How to get more from Teams

This is a flexible programme which will run in the morning and includes lunch to allow for various departure time in the afternoon.

Tony Savill, President, Molecular Dimensions Ltd.

Departures

4th Nanomem Course - Membrane Protein Crystallisation, Paul Scherrer Institute, CH

Membrane Protein Crystallisation – Serial Crystallography – Research Integrity

Program

3-days training at the Paul Scherrer Institute (PSI), CH-5232 Villigen with starting time Monday 24th November at 12.30 pm (get together lunch) and end Thursday 27th November at 1 pm.

Monday 24th November 2014

12.30 pm

meet at PSI-Ost (!not “West”) main entrance & get together lunch

@ Oase

14.00 pm

Guided tour of PSI Swiss Light (SLS), Neutron and Proton Sources by J.Galivano and T.Weinert

@ WBGB/20 (west side)

16.30 pm

Welcome Speech of LBR Director Kurt Ballmer and visit of LBR

OFLG first floor

18.30 pm

Welcome Apéro in the LBR coffee room

OFLG coffee room

     

Tuesday 25th November 2014

9.00 pm

Talk : “Lipidic cubic phase serial millisecond crystallography using synchrotron radiation” by Jörg Standfuss

OFLA /209

10.00 pm

Samples preparation for the beamtime(LCP syringes for SFX/SMX) with Przemek Nogly

OFLG / 101

11.40 pm

early Lunch

Oase

12.40

Pick samples and transfer to the west side

LBR -> SLS

13.00 pm

In situ diffraction at beamline PXIII

SLS

17.30 pm

Walk to Villigen (2km)

 
 

Dinner in Villigen: Gasthof Schlossberg

 
     

Wednesday 26th November 2014

9.00 pm

Talk : Research Integrity by Louis Tiefenauer

OFLG / 401

10.00pm

Workshop : science exchange and Virtual project discussion with Dmitry Veprintsev and Valérie Panneels

OFLG / 401

13.00 pm

Lunch

Oase

14.00 pm

Visit SwissFEL construction site: Ivo Widmer / Luc Patthey

SwissFEL

 

Break : group photo

 
 

Free evening /or/ Dinner at the Oase

Oase

Thursday 27th November 2014

9.00

Talk : “Fluorescence methods for pre-crystallization screening and quality control of membrane proteins” by Chayne Piscitelli

OFLA / 209

10.00

Talk : „Structural basis of GPCR activation“ by Xavier Deupi

OFLA / 209

11.00

Talk : “Lattice contacts and biological interfaces: understanding the fabric of protein (nano)-crystals” by Guido Capitani

OFLA / 209

12.00 pm

Lunch at the Oase

Oase

13.00 pm

Departure

 
     

-coordination: Valérie PanneelsThis email address is being protected from spambots. You need JavaScript enabled to view it.

List of Fellows

       

Robert Dods

Uni Goth, SE

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Katarina Jungnickel

Uni Oxford, UK

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Jonna Hakulinen

Astra Zeneca, SE

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Gabriela Kovácsová

MPI, DE

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Markus Metz

DESY, DE

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Ana Rita Santos Silva

Imperial College, UK

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Anastasya Shilova

ESRF, F

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Živa Vuckovic

SWISSCI/PSI, CH

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Aleksandra Woznicka

IBS, F

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Wenting Wu

PSI, CH

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Extern:

Pascelle Draper

SWISSCI

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Arrival and Accomodations

How to reserve a room at the PSI guesthouse:

http://www.psi.ch/gaestehaus/anmeldeformular

How to get to the Paul Scherrer Institute:

PSI is located in northern Switzerland, approximately midway between Zürich and Basel. The nearest towns and railway stations are Baden and Brugg. Frequent air and train connections via Zürich or Basel are available from all major European cities.

 

To find PSI by car

You can reach PSI via Brugg or Baden.

Via Brugg:
Follow the Koblenz–Zurzach signs through Brugg. After passing through Lauffohr and a short hill, branch off to the left towards Remigen/Villigen; then after about 500 m turn right towards Villigen. Approximately 1 km after leaving Villigen you will reach PSI-West. You can drive to PSI-East via the bridge over the river Aare.

Via Baden:
Follow the Koblenz–Zurzach signs through Baden. Drive through Nussbaumen, Untersiggenthal and Station Siggenthal. Approximately 1.5 km beyond the roundabout, follow the sign left towards PSI at the crossroads and you will reach PSI-East. You can drive to PSI-West via the bridge over the river Aare.

Programming of the navigation system:
For PSI West: enter city "Villigen", street "PSI"
For PSI East: enter city "Würenlingen", street "PSI"
It is permitted to use the connecting road across the river Aare.

To reach PSI by public transport

Brugg is on the train line (Zürich–Basel, Zürich–Bern). You can take a public bus (Postauto 376) from Brugg railway station. Take the Brugg–PSI–Böttstein–Döttingen bus, and within 20 minutes you will arrive at PSI.

When you land at the airport

There is an SBB railway station at the International Airport at Zürich-Kloten. Take the train to Brugg and a public bus (Postauto) from Brugg railway station to Villigen PSI.

 

 

 

 


 3rd NanoMem training, University of Gothenburg

Science and Society: The role of science in society, public outreach, career development and gender issues

 

Location

Seminar room: Swedish NMR center ground floor (Hasselblad Laboratory, Medicinaregatan 5C, Göteborg)

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Program

Thursday 5th December

08:00 - 08:30 Introduction

Richard Neutze

08:30 – 10:30 Media a threat or a possibility - keys to the media landscape and a successful interview

Claes Johnsson

10:30-11:00 Break, photography session

11:00-11:35 Public outreach in Chemistry

Kristina Hedfalk

11:35-12:00 DOs & DON'Ts when you are about to publish in a high-profile journal

Gergely Katona

12:00-13:00 Lunch

13:00-14:30 Rhetorics and how to give a presentation that works.

Einar Korpus

14:30-14:45 Break

14:45-16:15 Intellectual properties, commercialization

Gregory Carson

16:15-17:45 Intellectual properties, commercialization

Lorna Fletcher

Friday 6th December

09:15-10:45 Science, Society and Gender

Ulla Eriksson-Zetterquist

10:45-11:00 Break

11:00-12:00 Visit to the membrane protein facilities at the University

12:00-13:00 Lunch

13:00-16:00 An Introduction to Career Planning

Marie Hagman

James Dresch

16:00 - 17:00 Writing carrier development plan, collecting plans for secondments

Saturday 7th December

10:00 – 16:00 Visit to Universeum

 

 

 

2nd NanoMem Training

Drug Discovery from a Structure and Biophysics perspective

AstraZeneca

Program (All seminars in SC251 at AstraZeneca, Mölndal)

Day 1 (Monday December 2, 2013)

08:45                   Arrival to the main reception (KC)

09:00 – 09:30              Welcome and short introduction to AstraZeneca and the course (Margareta Ek)

09:30 – 10:00      Coffee

10:00 – 11:00      Pre-Target Selection to Lead Optimization Investment Decision (Judith Hartleib)


11:00 – 12:00      Lunch at AstraZeneca canteen

12:00 – 13:00      Biophysics (Stefan Geschwindner)

13:00 – 14:00      FBLG by Crystallography (Yafeng Xue)

14:00 – 15:00      Reagents and Assay Development (Niek Dekker)

15:00 – 15:30     Coffee

Day 2 (Tuesday December 3, 2013)

 

08:45                   Arrival to the main reception (KC)

09:00 – 10:00      Medicinal chemistry: LOID to CSID (Kenneth Granberg)

10:00 – 11:00      Compound Management (Hanna Jansson)

11:00 – 12:00      Lunch at AstraZeneca canteen

12:00 – 13:00      Guided tour at Compound management (Hanna Jansson)

13:00 – 14:00      NMR (Fredrik Edfeldt)

14:00 – 15:00      Isabel Moraes, MPL, Diamond Light Source

15:00 – 15:30      Coffee

 

Day 3 (Wednesday December 4, 2013)

 

 

08:45                   Arrival to the main reception (KC)

9:00 – 10:00                  The Drug Discovery process from Preclinical moving into man (Per-Erik Strömstedt)

10:00 – 11:00      HTS/Screening (Helen Boyd)

11:00 – 12:00      Lunch at AstraZeneca canteen

12:00 – 13:00      Pharmaceutical Development (Anna Pettersen)

13:00 – 14:00      Crystallography (Patrik Johansson)

14:00 – 14:30                Guided tour in the Protein Expression and Purification Lab (Jonna Hakulinen)

14:30 – 15:00                Guided tour in the Biophysics and Crystallography Lab (Jonna Hakulinen)

15:00 – 15:30      Coffee